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| Status |
Public on Jan 01, 2020 |
| Title |
The NAD kinase OsNADK1 affects the redox and enhances the tolerance to drought in rice |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
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| Summary |
NAD(H) and NADP(H) are crucial coenzymes and play important and distinguishable roles in all living organisms. NAD(H) is primarily involved in catabolic reactions, whereas NADP(H) participates in anabolic reactions, such as NADP(H)-dependent reductive anabolic pathways, signal transduction, and defense against oxidative stress. Hence, regulation of the intracellular balance of NAD(H) and NADP(H) is critical. NAD(H) kinase (NADK) is the key enzyme in the de novo biosynthesis of NADP(H), catalyzing the transfer of a phosphoryl group from ATP to NAD(H), and thus plays an important role in the regulation of intracellular NAD(H)/NADP(H) balance for NADP(H)-based metabolic pathways.
We used microarrays to detail the global programme of gene expression between WT and osnadk1 and identified distinct classes of up-regulated and down-regulated genes during this process.
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| Overall design |
The youngest fully expanded leaves from 2.5-month-old WT and osnadk1 plants (three replicates each), grown under normal growth (soil moisture, 47.3%) and drought conditions (soil moisture, 8.5%), were used for RNA extraction and hybridization on Affymetrix microarrays. Control: normal growth condition; Drought: drought stress condition.
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| Contributor(s) |
Wang X, Zhou C, Chen K |
| Citation(s) |
31910821 |
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| Submission date |
Jan 22, 2017 |
| Last update date |
Apr 01, 2020 |
| Contact name |
Kunming Chen |
| E-mail(s) |
[email protected]
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| Organization name |
Northwest A&F University
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| Department |
College of Life Sciences
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| Lab |
State Key Laboratory of Crop Stress Biology in Arid Area
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| Street address |
Xinong Road 22#
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| City |
Yangling |
| State/province |
Shaanxi |
| ZIP/Postal code |
712100 |
| Country |
China |
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| Platforms (1) |
| GPL2025 |
[Rice] Affymetrix Rice Genome Array |
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| Samples (12)
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| GSM2464979 |
osnadk1 mutant under normal growth condition, biological rep1 |
| GSM2464980 |
osnadk1 mutant under normal growth condition, biological rep2 |
| GSM2464981 |
osnadk1 mutant under normal growth condition, biological rep3 |
| GSM2464982 |
osnadk1 mutant under drought stress condition, biological rep1 |
| GSM2464983 |
osnadk1 mutant under drought stress condition, biological rep2 |
| GSM2464984 |
osnadk1 mutant under drought stress condition, biological rep3 |
| GSM2464985 |
WT plants under normal growth condition, biological rep1 [re-analysis] |
| GSM2464986 |
WT plants under normal growth condition, biological rep2 [re-analysis] |
| GSM2464987 |
WT plants under normal growth condition, biological rep3 [re-analysis] |
| GSM2464988 |
WT plants under drought stress condition, biological rep1 [re-analysis] |
| GSM2464989 |
WT plants under drought stress condition, biological rep2 [re-analysis] |
| GSM2464990 |
WT plants under drought stress condition, biological rep3 [re-analysis] |
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| Relations |
| BioProject |
PRJNA362829 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE93917_RAW.tar |
49.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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