|
| Status |
Public on Jan 19, 2018 |
| Title |
Pan-AcH4 ChIP-chip with High Resolution Tiling Arrays on HUVECs and HuAoVSMCs |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Differential EC enrichment of pan-AcH4 was assessed at 34 select genes, including 19 EC-enriched genes, 6 broadly expressed genes, and 9 EC-excluded genes, by pan-AcH4 (AcH4K5, AcH4K8, AcH4K12, and AcH4K16) ChIP-chip analysis on human umbilical vein endothelial cells (HUVECs) and human aortic vascular smooth muscle cells (HuAoVSMCs) with a high resolution tiling DNA microarray. Specifically, the tiling array analyzes the 50 kb genomic region upstream of transcription initiation, the intragenic regions , and 50 kb genomic region downstream of the last exon.
|
| |
|
| Overall design |
Pan-AcH4 ChIP was conducted on HUVECs and HuAoVSMCs. These ChIP samples were hybridized to a custom 1 M agilent microarray for ChIP-chip analysis.
|
| |
|
| Contributor(s) |
Marsden PA, Yan MS |
| Citation(s) |
29414790 |
| |
| Submission date |
Jan 23, 2017 |
| Last update date |
Apr 24, 2018 |
| Contact name |
Philip A Marsden |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Toronto
|
| Department |
Medicine
|
| Street address |
209 Victoria Street, Room 522
|
| City |
Toronto |
| ZIP/Postal code |
M5B 1C6 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL22952 |
Agilent-027044 Marsden Lab 1M array |
|
| Samples (6)
|
| GSM2465761 |
AcH4 Human Umbilical Vein Endothelial Cells 1 |
| GSM2465762 |
AcH4 Human Umbilical Vein Endothelial Cells 2 |
| GSM2465763 |
AcH4 Human Umbilical Vein Endothelial Cells 3 |
| GSM2465764 |
AcH4 Human Aortic Vascular Smooth Muscle Cells 1 |
| GSM2465765 |
AcH4 Human Aortic Vascular Smooth Muscle Cells 2 |
| GSM2465766 |
AcH4 Human Aortic Vascular Smooth Muscle Cells 3 |
|
| Relations |
| BioProject |
PRJNA362931 |
Less...
More...