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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 01, 2017 |
| Title |
GPR56/ADGRG1 inhibits Mesenchymal Differentiation and Radioresistance in Glioblastoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
A mesenchymal transition occurs both during natural evolution of glioblastoma (GBM) and in response to therapy. However, the molecular mechanisms underlying mesenchymal differentiation are not well understood. We have found that the adhesion G protein-coupled receptor, GPR56/ADGRG1, inhibits mesenchymal differentiation and radioresistance in glioblastoma stem-like initiating cells (GICs). Here, we have performed microarray analysis of control- versus GPR56 knockdown-GICs to characterize gene expression changes upon GPR56 knockdown and identify a gene expression signature associated to GPR56.
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| Overall design |
To identify a GPR56-associated gene signature we compared the gene expression profile of control- and GPR56 knockdown-GICs by genome-wide microarray analysis. Patient-derived CD44low GICs (control- and GPR56 knockdown-GICs) were cultured under adherent conditions on laminin in serum-free neural stem cell medium (in the presence of bFGF and EGF) at 37°C, 5% CO2 and 5% oxygen. Total RNA was extracted, labeled and hybridized in a SurePrint G3 Human GE 8x60k array (Agilent Technologies). Four paired biological replicates of each control- and GPR56 knockdown-GICs were used.
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| Contributor(s) |
de la Iglesia N |
| Citation(s) |
29166609 |
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| Submission date |
Feb 10, 2017 |
| Last update date |
Mar 27, 2018 |
| Contact name |
Nuria de la Iglesia |
| E-mail(s) |
[email protected]
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| Organization name |
IDIBAPS
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| Street address |
Rossello 153
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| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
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| Platforms (1) |
| GPL16699 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Feature Number version) |
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| Samples (8)
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| GSM2482358 |
GIC2, control shRNA, rep 2 |
| GSM2482359 |
GIC7, GPR56 shRNA, rep 1 |
| GSM2482360 |
GIC7, GPR56 shRNA, rep 2 |
| GSM2482361 |
GIC2, GPR56 shRNA, rep 1 |
| GSM2482362 |
GIC2, GPR56 shRNA, rep 2 |
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| Relations |
| BioProject |
PRJNA373987 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE94765_RAW.tar |
99.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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