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| Status |
Public on Apr 22, 2008 |
| Title |
HybridPoplar_H11-11_Leaves_MalacosomaDisstria_Herbivory |
| Platform organism |
Populus |
| Sample organism |
Populus trichocarpa x Populus deltoides |
| Experiment type |
Expression profiling by array
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| Summary |
From a set of 15 standard, normalized or full-length cDNA libraries, we generated 139 007 3'- or 5'-end sequenced ESTs, representing more than one-third of the c. 385 000 publicly available Populus ESTs. Clustering and assembly of 107 519 3'-end ESTs resulted in 14 451 contigs and 20 560 singletons, altogether representing 35 011 putative unique transcripts, or potentially more than three-quarters of the predicted c. 45 000 genes in the poplar genome. Using this EST resource, we developed a cDNA microarray containing 15 496 unique genes, which was utilized to monitor gene expression in poplar leaves in response to herbivory by forest tent caterpillars (Malacosoma disstria). After 24 h of feeding, 1191 genes were classified as up-regulated, compared to only 537 down-regulated. Functional classification of this induced gene set revealed genes with roles in plant defence (e.g. endochitinases, Kunitz protease inhibitors), octadecanoid and ethylene signalling (e.g. lipoxygenase, allene oxide synthase, 1-aminocyclopropane-1-carboxylate oxidase), transport (e.g. ABC proteins, calreticulin), secondary metabolism [e.g. polyphenol oxidase, isoflavone reductase, (–)-germacrene D synthase] and transcriptional regulation [e.g. leucine-rich repeat transmembrane kinase, several transcription factor classes (zinc finger C3H type, AP2/EREBP, WRKY, bHLH)]. This study provides the first genome-scale approach to characterize insect-induced defences in a woody perennial providing a solid platform for functional investigation of plant–insect interactions in poplar.
Keywords: stress response
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| Overall design |
Ten hybridizations were performed comparing FTC-treated poplar leaves after 24 h of continuous feeding and untreated control leaves harvested at the same time. RNA isolated from each of the five individual FTC-treated trees was compared directly against the five individual untreated control trees using two hybridizations with a dye flip for each tree pair.
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| Contributor(s) |
Ralph SG, Bohlmann J |
| Citation(s) |
16626454, 18230180 |
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| Submission date |
Nov 05, 2007 |
| Last update date |
Nov 12, 2019 |
| Contact name |
Joerg Bohlmann |
| E-mail(s) |
[email protected]
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| Phone |
604-822-0282
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| Organization name |
University of British Columbia
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| Department |
Michael Smith Laboratories
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| Lab |
Joerg Bohlmann
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| Street address |
#301 - 2185 East Mall
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| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6T 1Z4 |
| Country |
Canada |
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| Platforms (1) |
| GPL5921 |
Treenomix poplar 15.5K cDNA microarray |
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| Samples (10)
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| GSM234759 |
HybridPoplar_H11-11_Leaves_MalacosomaDisstria_HerbivoryHyb1 |
| GSM234763 |
HybridPoplar_H11-11_Leaves_MalacosomaDisstria_HerbivoryHyb2 |
| GSM234764 |
HybridPoplar_H11-11_Leaves_MalacosomaDisstria_HerbivoryHyb3 |
| GSM234765 |
HybridPoplar_H11-11_Leaves_MalacosomaDisstria_HerbivoryHyb4 |
| GSM234766 |
HybridPoplar_H11-11_Leaves_MalacosomaDisstria_HerbivoryHyb5 |
| GSM234767 |
HybridPoplar_H11-11_Leaves_MalacosomaDisstria_HerbivoryHyb6 |
| GSM234768 |
HybridPoplar_H11-11_Leaves_MalacosomaDisstria_HerbivoryHyb7 |
| GSM234769 |
HybridPoplar_H11-11_Leaves_MalacosomaDisstria_HerbivoryHyb8 |
| GSM234770 |
HybridPoplar_H11-11_Leaves_MalacosomaDisstria_HerbivoryHyb9 |
| GSM234771 |
HybridPoplar_H11-11_Leaves_MalacosomaDisstria_HerbivoryHyb10 |
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| Relations |
| BioProject |
PRJNA103327 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9522_RAW.tar |
433.8 Mb |
(http)(custom) |
TAR (of TIFF) |
| Processed data included within Sample table |
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