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Series GSE9525 Query DataSets for GSE9525
Status Public on Oct 01, 2008
Title Effect of short- and long-term morphine treatment on gene expression in the hypothalamus and pituitary
Organism Mus musculus
Experiment type Expression profiling by array
Summary Addictive drugs including opioids activate signal transduction pathways that regulate gene expression in the brain. However, changes in CNS gene expression following morphine exposure are poorly understood. We studied the effect of short- and long-term morphine treatment on gene expression in the hypothalamus and pituitary using genome-wide DNA microarray and real-time reverse transcriptase polymerase chain reaction (RT-PCR) analyses. In the hypothalamus, we found that short-term morphine administration up-regulated (at least 2-fold) 39 genes and down-regulated six genes. Long-term morphine administration up-regulated 35 genes and down-regulated 51 hypothalamic genes. In the pituitary, we found that short-term morphine administration up-regulated (at least 2-fold) 110 genes and down-regulated 29 genes. Long-term morphine administration up-regulated 85 genes and down-regulated 37 pituitary genes. Strikingly, microarray analysis uncovered several genes involved in food intake (neuropeptide Y, agouti-related protein, and cocaine and amphetamine-regulated transcript) whose expression was strongly altered by morphine exposure in either the hypothalamus or pituitary. Subsequent RT-PCR analysis confirmed similar gene regulation of noteworthy genes in these regions. Finally, we found functional correlation between morphine-induced alterations in food intake and regulations of genes involved in this process. Changes in genes related to food intake may uncover new pathways related to some of the physiological effects of opioids.
Keywords: Comparative treatment versus placebo
 
Overall design 8 samples analyzed: 4 from hypothalamus (2 biological replicates and 2 dye swaps) and 4 from pituitary (2 biological replicates and 2 dye swaps)
8 samples analyzed: 4 from hypothalamus short term treatment (2 biological replicates and 2 dye swaps) and 4 hypothalamus long term treatment (2 biological replicates and 2 dye swaps)
 
Contributor(s) Anghel A
Citation(s) 20144693
Submission date Nov 06, 2007
Last update date Jan 18, 2013
Contact name Adrian Anghel
E-mail(s) aanghel@mednet.ucla.edu
Phone 310-586-2084
Fax 419-791-2755
URL http://anghel.exofire.net/
Organization name University of California, Los Angeles (UCLA)
Department Medicine
Lab UCLA Translational Oncology Research Lab
Street address 2825 Santa Monica Blvd. Suite 200
City Santa Monica
State/province CA
ZIP/Postal code 90404-2429
Country USA
 
Platforms (1)
GPL6050 SCCPRR_Mouse_19549_v1.1
Samples (18)
GSM239721 Hypothalamus 4day(H1/L1)
GSM241212 Hypothalamus 4day(H1s/L1s);dye swap
GSM241213 Hypothalamus 4day(H2/L2);biological replicate
Relations
BioProject PRJNA103333

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9525_RAW.tar 25.3 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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