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Status |
Public on Oct 01, 2008 |
Title |
Effect of short- and long-term morphine treatment on gene expression in the hypothalamus and pituitary |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Addictive drugs including opioids activate signal transduction pathways that regulate gene expression in the brain. However, changes in CNS gene expression following morphine exposure are poorly understood. We studied the effect of short- and long-term morphine treatment on gene expression in the hypothalamus and pituitary using genome-wide DNA microarray and real-time reverse transcriptase polymerase chain reaction (RT-PCR) analyses. In the hypothalamus, we found that short-term morphine administration up-regulated (at least 2-fold) 39 genes and down-regulated six genes. Long-term morphine administration up-regulated 35 genes and down-regulated 51 hypothalamic genes. In the pituitary, we found that short-term morphine administration up-regulated (at least 2-fold) 110 genes and down-regulated 29 genes. Long-term morphine administration up-regulated 85 genes and down-regulated 37 pituitary genes. Strikingly, microarray analysis uncovered several genes involved in food intake (neuropeptide Y, agouti-related protein, and cocaine and amphetamine-regulated transcript) whose expression was strongly altered by morphine exposure in either the hypothalamus or pituitary. Subsequent RT-PCR analysis confirmed similar gene regulation of noteworthy genes in these regions. Finally, we found functional correlation between morphine-induced alterations in food intake and regulations of genes involved in this process. Changes in genes related to food intake may uncover new pathways related to some of the physiological effects of opioids. Keywords: Comparative treatment versus placebo
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Overall design |
8 samples analyzed: 4 from hypothalamus (2 biological replicates and 2 dye swaps) and 4 from pituitary (2 biological replicates and 2 dye swaps) 8 samples analyzed: 4 from hypothalamus short term treatment (2 biological replicates and 2 dye swaps) and 4 hypothalamus long term treatment (2 biological replicates and 2 dye swaps)
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Contributor(s) |
Anghel A |
Citation(s) |
20144693 |
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Submission date |
Nov 06, 2007 |
Last update date |
Jan 18, 2013 |
Contact name |
Adrian Anghel |
E-mail(s) |
aanghel@mednet.ucla.edu
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Phone |
310-586-2084
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Fax |
419-791-2755
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URL |
http://anghel.exofire.net/
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Organization name |
University of California, Los Angeles (UCLA)
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Department |
Medicine
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Lab |
UCLA Translational Oncology Research Lab
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Street address |
2825 Santa Monica Blvd. Suite 200
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City |
Santa Monica |
State/province |
CA |
ZIP/Postal code |
90404-2429 |
Country |
USA |
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Platforms (1) |
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Samples (18)
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GSM239721 |
Hypothalamus 4day(H1/L1) |
GSM241212 |
Hypothalamus 4day(H1s/L1s);dye swap |
GSM241213 |
Hypothalamus 4day(H2/L2);biological replicate |
GSM241214 |
Hypothalamus 4day(H2s/L2s);biological replicate_dye swap |
GSM241215 |
Pituitary 4day(P1) |
GSM241216 |
Pituitary 4day(P1s);dye swap |
GSM241217 |
Pituitary 4day(P2/L1);biological replicate |
GSM241218 |
Pituitary 4day(P2s/L1s);biological replicate_dye swap |
GSM241569 |
Hypothalamus 6hour(H1/S1) |
GSM241570 |
Hypothalamus 6hour(H1s/S1s);dye swap |
GSM241571 |
Hypothalamus 6hour (H2/S2);biological replicate |
GSM241572 |
Hypothalamus 6hour (H2s/S2s);biological replicate_dye swap |
GSM241573 |
Pituitary 6hour (P1/S1) |
GSM241574 |
Pituitary 6hour(P1s/S1s);dye swap |
GSM241575 |
Pituitary 6hour(P2/S2);biological replicate |
GSM241576 |
Pituitary 6hour (P2s/S2s);biological replicate_dye swap |
GSM241979 |
Pituitary 4day(L2);biological replicate |
GSM241980 |
Pituitary 4day(L2s);biological replicate_dye swap |
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Relations |
BioProject |
PRJNA103333 |