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| Status |
Public on Mar 02, 2017 |
| Title |
Transgenic Plasmodium falciparum blood stage parasites F12/GDV1-GFP-DD: control (-Shield-1;F12/GDV1-GFP-DDOFF) vs GDV1-overexpressing (+Shield-1;F12/GDV1-GFP-DDON) parasites |
| Platform organism |
Plasmodium falciparum |
| Sample organisms |
Plasmodium falciparum; Plasmodium falciparum 3D7 |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptional profiling of transgenic P. falciparum blood stage parasites expressing an ectopic copy of GDV1-GFP-DD (F12/GDV1-GFP-DD). The DD (FKBP destabilisation domain) allows for the conditional expression of fusion proteins: DD fusion proteins are rapidly degraded or stably expressed in absence or presence of the stabilising ligand Shield-1, respectively (Banaszynski LA, Chen LC, Maynard-Smith LA, Ooi AG, Wandless TJ. A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules.Cell. 2006 Sep 8;126(5):995-1004). The goal of this experiment was to identify genes differentially expressed in response to induced GDV1 overexpression.
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| Overall design |
P. falciparum clone F12 parasites carrying the pHcam_GDV1-GFP-DD episome were grown in presence of 4nM WR (F12/GDV1-GFP-DDOFF) or 4nM WR/312nM Shield-1 (F12/GDV1-GFP-DDON). RNA extracted from these samples at seven consecutive time points each was processed for microarray analysis.
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| Contributor(s) |
Filarsky M, Fraschka S, Carrington E, Niederwieser I, Moes S, Jenoe P, Bartfai R, Voss TS |
| Citation(s) |
29590075 |
| |
| Submission date |
Mar 01, 2017 |
| Last update date |
Jan 24, 2019 |
| Contact name |
Till Steffen Voss |
| E-mail(s) |
[email protected]
|
| Phone |
+41612848161
|
| Organization name |
Swiss Tropical and Public Health Institute
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| Department |
Medical Parasitology and Infection Biology
|
| Lab |
Malaria Gene Regulation
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| Street address |
Socinstrasse 59
|
| City |
Basel |
| ZIP/Postal code |
4051 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL15130 |
Agilent-037237 P.falciparum_Pf3D7v7.1_Transcriptome_apimito |
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| Samples (14)
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| GSM2516170 |
F12/GDV1-GFP-DDOFF generation 1 24-32hpi |
| GSM2516171 |
F12/GDV1-GFP-DDOFF generation 1 32-40hpi |
| GSM2516172 |
F12/GDV1-GFP-DDOFF generation 1 40-48hpi |
| GSM2516173 |
F12/GDV1-GFP-DDOFF generation 2 8-16hpi |
| GSM2516174 |
F12/GDV1-GFP-DDOFF generation 2 16-24hpi |
| GSM2516175 |
F12/GDV1-GFP-DDOFF generation 2 24-32hpi |
| GSM2516176 |
F12/GDV1-GFP-DDOFF generation 2 32-40hpi |
| GSM2516177 |
F12/GDV1-GFP-DDON generation 1 24-32hpi |
| GSM2516178 |
F12/GDV1-GFP-DDON generation 1 32-40hpi |
| GSM2516179 |
F12/GDV1-GFP-DDON generation 1 40-48hpi |
| GSM2516180 |
F12/GDV1-GFP-DDON generation 2 8-16hpi |
| GSM2516181 |
F12/GDV1-GFP-DDON generation 2 16-24hpi |
| GSM2516182 |
F12/GDV1-GFP-DDON generation 2 24-32hpi |
| GSM2516183 |
F12/GDV1-GFP-DDON generation 2 32-40hpi |
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| This SubSeries is part of SuperSeries: |
| GSE95549 |
Transcriptional response to conditional over-expression of GDV1 in Plasmodium falciparum 3D7 wild type and F12 mutant parasites |
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| Relations |
| BioProject |
PRJNA377429 |
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