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| Status |
Public on Nov 13, 2017 |
| Title |
Understanding lipid metabolism in high-lipid-producing Chlorella vulgaris mutants at the genome-wide level |
| Organism |
Chlorella vulgaris |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study, physical and chemical mutagenesis methods were applied to enhance lipid productivity in Chlorella vulgaris. Then, de novo RNA-seq was performed to observe lipid metabolism changes at the genome-wide level. Characterization of two mutants, UV-715 and EMS-25, showed marked increases in lipid contents, i.e., 42% and 45%, respectively. In addition, the biomass productivity of the UV-715 cells was 9% higher than that of wild-type cells. Furthermore, gas chromatography-mass spectrophotometry analysis showed that both mutants have higher fatty acid methyl ester (FAME) contents than wild-type cells. To understand the effect of mutations that caused yield changes in UV-715 and EMS-25 cells at a genome-wide level, we carried out de novo RNA-seq. As expected, the transcriptional levels of the lipid biosynthesis genes were up-regulated, while the transcriptional levels of genes involved in lipid catabolism were down-regulated. Surprisingly, the transcriptional levels of the genes involved in nitrate assimilation and detoxification of reactive oxygen species (ROS) were significantly increased in the mutants. The genome-wide analysis results highlight the importance of nitrate metabolism and detoxification of ROS for high biomass and lipid productivity.
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| Overall design |
mRNA profiles of wild type and mutant (UV-715 and EMS-25) Chlorella vulgaris SAG 211-12 cells were generated at stationary phase cell culture by deep sequencing, in two biological replicates, using Illumina MiSeq platform.
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| Contributor(s) |
Tardu M, Sarayloo E, Kavakli IH |
| Citation |
Ehsan Sarayloo, Mehmet Tardu, Yigit Sabri Unlu, Salim Simsek, Gul Cevahir, Can Erkey, Ibrahim Halil Kavakli. Understanding lipid metabolism in high-lipid-producing Chlorella vulgaris mutants at the genome-wide level. Algal Research 2017;28:244-252. DOI:10.1016/j.algal.2017.11.009
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| Submission date |
Mar 06, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
mehmet tardu |
| E-mail(s) |
[email protected]
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| Organization name |
University of Michigan
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| Department |
Chemistry
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| Lab |
Koutmou Lab
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| Street address |
930 N University Ave
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
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| Platforms (1) |
| GPL23151 |
Illumina MiSeq (Chlorella vulgaris) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA378177 |
| SRA |
SRP101426 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE95708_Trinity_C_vulgaris.fasta.gz |
6.8 Mb |
(ftp)(http) |
FASTA |
| GSE95708_trans_counts.TMM.EXPR.matrix.txt.gz |
384.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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