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| Status |
Public on Mar 11, 2017 |
| Title |
Probing Plasmodium falciparum sexual differentiation at the single cell level |
| Organism |
Plasmodium falciparum NF54 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Malaria parasites go through major transitions during their complex life cycle, yet the underlying differentiation pathways remain obscure. Here we apply single cell transcriptomics to unravel events that initiate sexual development in preparation for transmission of the parasite from human to mosquito. This proof-of-concept study provides a template to capture transcriptional diversity in heterogeneous parasite populations, with major implications for our understanding of parasite biology and the ongoing malaria elimination campaign.
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| Overall design |
Plasmodium-infected human erythrocytes were single-cell sorted into 384-well plates at three separate time points after LysoPC depletion gametocyte induction (4, 8, 12 hours post induction). Two rows (total of 48 wells) of each 384-well plate were reserved for control samples (no induction). A total of 1152 wells were sequenced, with 144 control wells, and 1008 induced wells.
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| Contributor(s) |
Brancucci NM, De Niz M, Straub TJ, Ravel D, Hitz E, Boltryk SD, Niederwieser I, Birren BW, Voss TS, Neafsey DE, Marti M |
| Citation(s) |
30320226 |
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| Submission date |
Mar 10, 2017 |
| Last update date |
Aug 05, 2019 |
| Contact name |
Daniel E. Neafsey |
| E-mail(s) |
[email protected]
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| Organization name |
Broad Institute
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| Street address |
415 Main St.
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
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| Platforms (1) |
| GPL23997 |
Illumina NextSeq 500 (Plasmodium falciparum NF54) |
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| Samples (1155)
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| Relations |
| BioProject |
PRJNA378758 |
| SRA |
SRP101718 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE96066_38hpi.filtered.tsv.gz |
86.7 Kb |
(ftp)(http) |
TSV |
| GSE96066_38hpi.normalized.tsv.gz |
121.3 Kb |
(ftp)(http) |
TSV |
| GSE96066_38hpi.raw.tsv.gz |
177.2 Kb |
(ftp)(http) |
TSV |
| GSE96066_42hpi.filtered.tsv.gz |
187.9 Kb |
(ftp)(http) |
TSV |
| GSE96066_42hpi.normalized.tsv.gz |
273.8 Kb |
(ftp)(http) |
TSV |
| GSE96066_42hpi.raw.tsv.gz |
270.9 Kb |
(ftp)(http) |
TSV |
| GSE96066_46hpi.filtered.tsv.gz |
230.5 Kb |
(ftp)(http) |
TSV |
| GSE96066_46hpi.normalized.tsv.gz |
343.2 Kb |
(ftp)(http) |
TSV |
| GSE96066_46hpi.raw.tsv.gz |
317.2 Kb |
(ftp)(http) |
TSV |
| GSE96066_all.normalized.tsv.gz |
720.8 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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