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| Status |
Public on Sep 06, 2008 |
| Title |
rlm1 mutant 3 hour exposure to zymolyase |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
We did transcription profiling on the effect of rlm1 deletion, gene involved in cell wall stress response
Keywords: cell wall stress response
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| Overall design |
S. cerevisiae (rlm1 mutant) was grown on YEPD. For Zymolyase experiments, yeast cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h 30min. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with 5units/ml Zymolyase 100T. Cells were collected at 3 hours of growth, frozen at -80 °C and processed for RNA extraction. The total RNA from two different cultures was analyzed, and, in addition, for each sample two different hybridizations were performed, including fluorochrome swapping in order to minimize transcriptional changes due to technical variability. This therefore corresponded to four DNA microarrays analyzed for each condition.
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| Contributor(s) |
Garcia R, Bermejo C, Rodriguez-Peña JM, Nombela C, Arroyo J |
| Citation(s) |
19234305 |
| |
| Submission date |
Dec 18, 2007 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Javier Arroyo |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
0034-913941746
|
| URL |
http://ucm.es/info/mfar
|
| Organization name |
Facultad de Farmacia (UCM)
|
| Department |
Microbiologia II
|
| Lab |
U4-Javier Arroyo
|
| Street address |
Plaza Ramon y Cajal S/N
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL7054 |
yeast48canada (condensed version) |
|
| Samples (4)
|
| GSM249105 |
rlm1 mutant 3 hours exposure to zymolyase 1 |
| GSM249106 |
rlm1 mutant 3 hours exposure to zymolyase 2 |
| GSM249125 |
rlm1 mutant 3 hours exposure to zymolyase 3 |
| GSM249126 |
rlm1 mutant 3 hours exposure to zymolyase 4 |
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| This SubSeries is part of SuperSeries: |
| GSE12684 |
hog1, slt2, rlm1, and msn2/4 mutants exposed to zymolyase |
|
| Relations |
| BioProject |
PRJNA114689 |
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