|
| Status |
Public on Jun 10, 2008 |
| Title |
Tobramycin Treatment of P. aeruginosa Biofilms Grown on CFBE41o- Cells |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by array
|
| Summary |
We grew Pseudomonas aeruginosa biofilms on CFBE41o- human airway cells in culture, and we treated these biofilms with tobramycin. Microarray analysis was performed to gain an understanding of the global transcriptional changes that occur during antibiotic treatment.
Keywords: Antibiotic Response
|
| |
|
| Overall design |
We compared three untreated control samples with three tobramycin-treated samples. Biofilms were grown on CFBE41o- cells in culture for 9 hours in MEM/0.4% arginine. Replicate samples were then incubated in the presence or absence of 500 μg/mL tobramycin for 30 minutes.
|
| |
|
| Contributor(s) |
Anderson GG, Moreau-Marquis S, Stanton BA, O'Toole GA |
| Citation(s) |
18212077 |
| |
| Submission date |
Dec 20, 2007 |
| Last update date |
Jul 06, 2016 |
| Contact name |
George O'Toole |
| E-mail(s) |
George.A.O'[email protected]
|
| Organization name |
Dartmouth Medical School
|
| Department |
Department of Microbiology and Immunology
|
| Street address |
505 Vail Building
|
| City |
Hanover |
| State/province |
NH |
| ZIP/Postal code |
03755 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL84 |
[Pae_G1a] Affymetrix Pseudomonas aeruginosa Array |
|
| Samples (6)
|
| GSM252496 |
P.aeruginosaCFBEbiofilm_untreated_Sample1 |
| GSM252501 |
P.aeruginosaCFBEbiofilm_untreated_Sample2 |
| GSM252505 |
P.aeruginosaCFBEbiofilm_untreated_Sample3 |
| GSM252506 |
P.aeruginosaCFBEbiofilm_Tb-treated_Sample1 |
| GSM252507 |
P.aeruginosaCFBEbiofilm_Tb-treated_Sample2 |
| GSM252508 |
P.aeruginosaCFBEbiofilm_Tb-treated_Sample3 |
|
| This SubSeries is part of SuperSeries: |
| GSE10030 |
Tobramycin Treatment of Pseudomonas aeruginosa |
|
| Relations |
| BioProject |
PRJNA109057 |
Less...
More...