|
| Status |
Public on Oct 18, 2013 |
| Title |
2457T_invasion |
| Sample type |
RNA |
| |
|
| Source name |
invasion growth
|
| Organism |
Shigella flexneri 2a str. 2457T |
| Characteristics |
genotype/variation: wild type
|
| Growth protocol |
intracellular bacteria, 3 hours post invasion of HCT-8 cell (colonic epithelial cell line)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
phenol/chlorofom followed by LiCl precipitation. Eukaryotic RNA was removed with MICROBEnrich kit (invitrogen)
|
| Label |
biotin
|
| Label protocol |
All protocols were done according to manufacturer's specifications: http://www.affymetrix.com/support/technical/manual/expression_manual.affx
|
| |
|
| Hybridization protocol |
All protocols were done according to manufacturer's specifications: http://www.affymetrix.com/support/technical/manual/expression_manual.affx
|
| Scan protocol |
All protocols were done according to manufacturer's specifications: http://www.affymetrix.com/support/technical/manual/expression_manual.affx
|
| Description |
SAMPLE2
|
| Data processing |
Data has not been processed other than those protocols within GCOSv1.4
|
| |
|
| Submission date |
Sep 13, 2012 |
| Last update date |
Oct 18, 2013 |
| Contact name |
David A Rasko |
| E-mail(s) |
[email protected]
|
| Phone |
410 706 6774
|
| Organization name |
University of Maryland School of Medicine
|
| Department |
Istitute for Genome Sciences/Microbiology and Immunology
|
| Street address |
801 W. Baltimore Street, Room 619
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21201 |
| Country |
USA |
| |
|
| Platform ID |
GPL13175 |
| Series (1) |
| GSE40851 |
Expression data from wild type and deltaS4473 Shigella flexneri 2457T |
|
