strain: C57BL/6J genotype/variation: WT gender: Male age: embryonic day 15 tissue: frontal cortex
Treatment protocol
Mouse frontal cortex was sampled from embryonic day 15 transgenic animals and littermates control using disecction kits.
Growth protocol
Mice harboring a Cyfip1 spanning BAC clone (RP24-333C15) were generated in the UCLA transgenic core (http://tmc.ctrl.ucla.edu/tg-core/) by injection into C57BL/6J pronuclei. Transgenic mice were identified by PCR screening of tail derived DNA.
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated from embryonic day 15 frontal cortex from transgenic animals (n=3) and littermates controls (n=3) using the Qiagen RNeasy Kit. RNA quantity and quality was assessed using a Nanodrop (Nanodrop Technologies) and the Agilent Bioanalyzer (Agilent Technologies), respectively. Total RNA (200ng) was amplified, biotinylated and hybridized on Illumina Mouse ref-8 v2.0 BeadChip arrays, querying the expression of ~25,600 well-annotated Refseq transcripts, as per manufacturer's protocol.
