|
| Status |
Public on Nov 24, 2009 |
| Title |
BV-5h-rep1-A |
| Sample type |
RNA |
| |
|
| Source name |
Human foreskin fibroblast, B virus, 5 hour
|
| Organism |
Homo sapiens |
| Characteristics |
B virus strain:E2490
|
| Biomaterial provider |
Julia K Hilliard
|
| Treatment protocol |
Human foreskin fibroblast (HFF) cells (ATCC, CRL-2097) were infected with B virus at m.o.i. of 10 at 4ºC for 1 hour and 37ºC for 4 hours.
|
| Growth protocol |
HFF cells were grown in Eagle's mimimum essential medium with 0.1mM non-essential amino acids, 1.0mM sodium pyruvate and Earle's BBS, 90%; fetal bovine serum, 10%.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by QIAGEN's RNeasy Total RNA Isolation kit according to the manufacturer's instructions.Two-cycle cDNA synthesis were performed using SuperScript Choice system(Invitrogen Life Technologies) according to manufacturer’s instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was generated by Enzo BioArray High Yield RNA transcript Labeling Kit (Affymetrix) according to manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Fragmented cRNA was hybridized to HG-U133A chip at 45º for 16 hrs. Following hybridization, microarry was washed and stained with strepavidin-conjugated phycoerythrin (SAPE) by using Affymetrix GeneChip Fluidic Station 400. Staining intensity was amplified by using a biotinlylated anti-streptavidin antibody followed by a second SAPE stain.
|
| Scan protocol |
Microarray was scanned by Agilent G2500A GeneArray Scanner.
|
| Description |
Gene expression data from B virus 5 hour post infection.
|
| Data processing |
Data was analyzed with GeneChip Operating Software 1.4 using Affymetrix default analysis setting and global scaling (scaling factor of 500) as normalization method.
|
| |
|
| Submission date |
Mar 16, 2006 |
| Last update date |
Nov 24, 2008 |
| Contact name |
Julia K Hilliard |
| E-mail(s) |
[email protected]
|
| Phone |
404-4136560
|
| Fax |
404-4136560
|
| Organization name |
Georgia State University
|
| Department |
Biology
|
| Lab |
Viral Immunology Center
|
| Street address |
50 Decatur St
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30303 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE4521 |
B virus and HSV-1 Infected Human Foreskin Fibroblast (HFF) expression profiles |
|
