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Sample GSM100953 Query DataSets for GSM100953
Status Public on Apr 01, 2006
Title PND28 Rat Testis_Rat230-2_12hr Corn Oil_Animal#62_04005
Sample type RNA
 
Source name Sample Type: tissue, Tissue: testis
Organism Rattus norvegicus
Characteristics Array Type: Rat230_2
Amount to Core: 10
Units of Amount: ug
Ratio: 2.0
RNA Integrity Number: 9.5
Bioanalyzer Results: excellent
Strain or Line: Fischer
Individual: 62
Sacrifice Method: CO2
Age: 28
Units of Age: PND
Biomaterial provider Kamin Johnson, CIIT Centers for Health Research, 6 Davis Drive, RTP, NC, 27709, Stephanie Lahousse, [email protected], 919-558-1267
Treatment protocol in vivo Treatment: Corn Oil, Treatment Duration: 12, Units of Duration: hours, Treatment Time: 6:35, Units of Treatment Time: am, Vehicle for Chemical: corn oil, Amount of Vehicle: 0.245, Route of Exposure: oral gavage, Units of Amount of Vehicle: ml, Dose or Concentration: 1, Units of Dose or Concentration: gm/kg bodyweight
Extracted molecule total RNA
Extraction protocol Extraction Method: Trizol
Additional Purification Method: Qiagen RNeasy Maxi Kit
Label biotin
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description KJ_0_12_62_Testis_Control_Rat230_2_04005: PND 28 Fischer rats were gavaged with a single dose of 1 gram/kg MEHP in corn oil or corn oil alone. The animals were sacrified 1, 2, 3, 6, or 12 hours after dosing. The testis were removed and RNA extracted using the QIAGEN RNeasy Maxi kit
Data processing Affymetrix GCOS 1.0
 
Submission date Mar 17, 2006
Last update date Mar 22, 2006
Contact name Kamin Johnson
E-mail(s) [email protected]
Phone 919-558-1439
Organization name CIIT Centers for Health Research
Department Biological Sciences
Street address 6 Davis Drive
City Research Triangle Park
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL1355
Series (1)
GSE4514 Microarray analysis of MEHP gavaged PND28 male rats

Data table header descriptions
ID_REF Affymetrix probe name
VALUE GCOS-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 701.398 P 0.000127
AFFX-BioB-M_at 1234.58 P 4.4e-05
AFFX-BioB-3_at 755.252 P 4.4e-05
AFFX-BioC-5_at 1556.76 P 5.2e-05
AFFX-BioC-3_at 1543.69 P 4.4e-05
AFFX-BioDn-5_at 5365.19 P 4.4e-05
AFFX-BioDn-3_at 9560.22 P 4.4e-05
AFFX-CreX-5_at 21924.9 P 5.2e-05
AFFX-CreX-3_at 24193.4 P 4.4e-05
AFFX-r2-Ec-bioB-5_at 881.869 P 0.000244
AFFX-r2-Ec-bioB-M_at 1125.82 P 0.000244
AFFX-r2-Ec-bioB-3_at 969.6 P 0.000244
AFFX-r2-Ec-bioC-5_at 1763.94 P 0.000244
AFFX-r2-Ec-bioC-3_at 1839.32 P 0.000244
AFFX-r2-Ec-bioD-5_at 11793.9 P 0.000244
AFFX-r2-Ec-bioD-3_at 12649.9 P 0.000244
AFFX-r2-P1-cre-5_at 25638 P 0.000244
AFFX-r2-P1-cre-3_at 34838.8 P 0.000244
AFFX-r2-Bs-lys-M_at 16.8062 P 0.046143
AFFX_Rat_GAPDH_5_at 6571.87 P 7e-05

Total number of rows: 31099

Table truncated, full table size 906 Kbytes.




Supplementary data files not provided

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