|
| Status |
Public on Oct 18, 2012 |
| Title |
Day20_A |
| Sample type |
RNA |
| |
|
| Source name |
Day20
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Peripheral blood
culture condition: CD138+ Plasma cells sorted on CD138 and 7-AAD, generated in the upper compartment of a transwell system after 7 days of IL-6, IFN-α and supplemented media, with M2-10B4 in the lower compartment.
gender: Male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol and amplified using Illumina® TotalPrepTM-96 RNA Amplification Kit
|
| Label |
Biotin
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina protocol
|
| Scan protocol |
Arrays were scanned on a BeadArray reader
|
| Data processing |
Data were quantile normalised using Lumi for R [64-bit R version 2.15.1 (2012-06-22), lumi version 2.8.0].
|
| |
|
| Submission date |
Sep 27, 2012 |
| Last update date |
Oct 18, 2012 |
| Contact name |
Matthew Anthony Care |
| E-mail(s) |
[email protected]
|
| Organization name |
The University of York
|
| Department |
Department of Biology
|
| Lab |
Bioscience Technology Facility, Data Science Hub
|
| Street address |
Wentworth Way
|
| City |
York |
| State/province |
North Yorkshire |
| ZIP/Postal code |
YO10 5DD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE41208 |
In vitro generation of long-lived human Plasma Cells |
|
