|
| Status |
Public on Oct 01, 2012 |
| Title |
Healthy individual of twinpair #2 |
| Sample type |
RNA |
| |
|
| Source name |
primary mucosal tissue, colon
|
| Organism |
Homo sapiens |
| Characteristics |
twin pair: #2
individual: healthy individual, non-inflamed
tissue: primary mucosal tissue, colon
mean age: 31.5
|
| Treatment protocol |
n/a
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted using RNEasy (Qiagen) according to the manufacturers guidelines
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45C on GeneChip HG U133 2.0 Plus Array (Affymetrix GeneChipHybridization Oven 640). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from whole biopsies, primary mucosal tissue
|
| Data processing |
Sample data was processed using GCOS 1.3.0 (Affymetrix default settings) and normalized using GCRMA (R Bioconductor, gcrma package).
|
| |
|
| Submission date |
Oct 01, 2012 |
| Last update date |
Oct 01, 2012 |
| Contact name |
Robert Haesler |
| E-mail(s) |
[email protected]
|
| Phone |
+49 (0) 431 597 5754
|
| Organization name |
University Clinic of Schleswig Holstein
|
| Department |
IKMB / 1st Dept. of Medicine
|
| Lab |
Functional Genomics Platform
|
| Street address |
Schittenhelmstr. 12
|
| City |
Kiel |
| ZIP/Postal code |
24105 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE22619 |
Genome wide expression data from discordant twins (ulcerative colitis, primary mucosal tissue) |
|
