|
| Status |
Public on Feb 13, 2007 |
| Title |
Saline, replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Saline treated macaque prefrontal cortex
|
| Organism |
Macaca fascicularis |
| Characteristics |
State: Presymptomatic
Tissue: Prefrontal cortex
Treatment: Saline
|
| Biomaterial provider |
Shared Ltd.
|
| Treatment protocol |
Treated daily (9:00 am) with MPTP hydrochloride (0.2 mg/kg, i.v., Sigma, St. Louis, MO) dissolved in saline. Sacrificed in ketamine anesthesia by sodium pentobarbital overdose
|
| Growth protocol |
Animals were housed in individual primate cages under controlled conditions of humidity (50 F 5%), temperature (24 F 18C) and light (12h light/12h dark cycles, time lights on 8:00 am), food and water were available ad libitum and animal care was supervised by veterinarians skilled in the healthcare and maintenance of nonhuman primates. Experiments were carried out in accordance with European Communities Council Directive of 24 November 1986 (86/609/EEC) for care of laboratory animals.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA from the prefrontal cortex sample was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
According to the standard protocol outlined in the Affymetrix GeneChip Expression Analysis Technical Manual
|
| |
|
| Hybridization protocol |
According to the manufacturer's instructions.
|
| Scan protocol |
At 3-Am resolution using the GeneChip System confocal scanner (Hewlett-Packard, Santa Clara, CA) with a 488-nm emission and detection at 70 nm, controlled by Affymetrix Microarray Suite 5.0 (Affymetrix).
|
| Description |
Prefrontal cortex of macaque, saline treatment, replicate 1.
|
| Data processing |
Raw
|
| |
|
| Submission date |
Mar 27, 2006 |
| Last update date |
Oct 29, 2008 |
| Contact name |
Markus Storvik |
| Organization name |
University of Eastern Finland
|
| Department |
Pharmacology
|
| Street address |
University of Eastern Finland
|
| City |
Kuopio |
| ZIP/Postal code |
70211 |
| Country |
Finland |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE4550 |
Genes regulated in MPTP-treated macaques |
|
