|
| Status |
Public on Nov 12, 2012 |
| Title |
colonic CD56+NKp46+CD117-CD127- cells_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
healthy colonic NK cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: colon
cell type: CD56+NKp46+CD117-CD127- cells
|
| Treatment protocol |
Colonic hematopoietic cells were isolated from human healthy colon fragment upon enzymatic digestion with Collagenase VIII (Sigma).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sorted cells were immediately lysed in RLT buffer supplemented with 10% beta-mercaptoethanol (Quiagen, France). Lysates of three distinct donors were pooled and RNA was isolated by using RNAeasy Microkit (Quiagen, France).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared following the double amplification protocol of MessagaAmp II aRNA Amplification Kit (Ambion).
|
| |
|
| Hybridization protocol |
cRNA were hybridized on Human Genome HG_U133 +2.0 Affymetrix chips according to manufacturer protocol.
|
| Scan protocol |
Chip images were generated using Affymetrix AGCC version 3.2 software.
|
| Description |
Gene expression data from human healthy colonic NK cells
EVR_R10-D1_U133_2
|
| Data processing |
Expression data were obtained using Affymetrix Expression Console software (version 1.1).
|
| |
|
| Submission date |
Oct 10, 2012 |
| Last update date |
Nov 12, 2012 |
| Contact name |
Thien-Phong Vu Manh |
| Organization name |
CIML
|
| Street address |
163 avenue de Luminy
|
| City |
marseille |
| ZIP/Postal code |
13288 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE41469 |
Mapping and genome-wide profiling of human NKp46+ cells |
|
