|
| Status |
Public on Nov 02, 2012 |
| Title |
rd1_P2_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
rd1 mouse retina at age postnatal day 2
|
| Organism |
Mus musculus |
| Characteristics |
tissue: retina
age: postnatal day 2
genotype: rd1
|
| Growth protocol |
Animals were grown to postnatal day 2, 4, 6 or 8 where eyes were enucleaded and retinas isolated. 8-14 retinas were combined per sample.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from retinas using TRIzol reagent according to manufacture's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip MOE430A arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetric GeneArray 2500 scanner.
|
| Data processing |
Expression scores were obtained using Robust Multichip Average to do background correction, quantile normalization and summarization. Normalized data was subjected to a two-stage analysis based on FDR-CI.
|
| |
|
| Submission date |
Oct 24, 2012 |
| Last update date |
Nov 02, 2012 |
| Contact name |
Matthew J Brooks |
| E-mail(s) |
[email protected]
|
| Phone |
301-443-4906
|
| Organization name |
NIH
|
| Department |
NEI
|
| Lab |
NNRL
|
| Street address |
6 Center Dr Bldg 6, Rm 303
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE41821 |
A role for Prenylated rab acceptor 1 in vertebrate photoreceptor development |
|
