|
| Status |
Public on Sep 05, 2013 |
| Title |
WTPB1 S3-control-replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Seed
|
| Organism |
Oryza sativa |
| Characteristics |
variety: PB1
genotype/variation: wild type
tissue: seed
developmental stage: S3 Stage (5-10 Days after pollination)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500 ng total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45˚C on GeneChip Arabidopsis Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Gene Chip Scanner 3300
|
| Description |
Gene expression data of wild type seed 5-10 DAP
|
| Data processing |
First-order analysis was performed using Affymetrix® GeneChip® Command Console® 3.0. Normalization of data and differential expression analysis was done using ArrayAssist ver 5.5.1 (Stratagene, CA, USA)
|
| |
|
| Submission date |
Nov 05, 2012 |
| Last update date |
Sep 05, 2013 |
| Contact name |
Sanjay Kapoor |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Delhi, South Campus
|
| Department |
Department of Plant Molecular Biology
|
| Street address |
Benito Huarez MArg
|
| City |
New Delhi |
| ZIP/Postal code |
110021 |
| Country |
India |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE42029 |
Silencing of OsMADS29 in Oryza sativa var. PB1 |
|
