|
| Status |
Public on Jan 12, 2015 |
| Title |
Control_ZT3(2) |
| Sample type |
RNA |
| |
|
| Source name |
Control Flies collected at ZT3
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: head
RNAi: control
circadian timepoint: ZT3
|
| Treatment protocol |
Female fly heads were collected and RNA prepared using zymo RNA purification kit
|
| Growth protocol |
Fly head collections of control and tim-fbl flies growth at 25C and collected at two different circadian timepoints.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Zymo RNA purification kit was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression from control flies collected at ZT3
|
| Data processing |
The data were analyzed with R suite using RMA
|
| |
|
| Submission date |
Nov 05, 2012 |
| Last update date |
Jan 12, 2015 |
| Contact name |
Sebastian Kadener |
| E-mail(s) |
[email protected]
|
| Phone |
++972-2-6585099
|
| Fax |
++972-2-6585118
|
| Organization name |
The Hebrew University of Jerusalem
|
| Department |
Biological Chemistry
|
| Street address |
The Alexander Silberman Inst. of Life Sciences, Edmond J. Safra Campus
|
| City |
Jerusalem |
| ZIP/Postal code |
91904 |
| Country |
Israel |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE42037 |
Comparison of Control and Tim-Fbl gene expression profiles |
|
