|
| Status |
Public on Mar 24, 2015 |
| Title |
blood sample_LD676-01b |
| Sample type |
RNA |
| |
|
| Source name |
leukocyte
|
| Organism |
Homo sapiens |
| Characteristics |
dx (diagnosis): ASD
gender: male
cell type: leukocyte
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted following standard procedures and manufacturer's instructions (Ambion, Austin, TX, USA). In principle, LeukoLOCK disks were freed from RNAlater and Tri-reagent was used to flush out the captured lymphocytes and lyse the cells. RNA was subsequently precipitated with ethanol and purified though washing and cartridge-based steps.
|
| Label |
Biotin
|
| Label protocol |
11 uL of each sample for RNA amplification which was performed using the Illumina TotalPrep RNA Amplification Kit. Elution in 40uL of water and then diluted samples to 150ng/uL.
|
| |
|
| Hybridization protocol |
5uL was used in the Illumina Whole Genome Gene Expression Direct Hybridization Protocol
|
| Scan protocol |
Illumina BeadArray Reader
|
| Data processing |
Illumina GenomeStudio® software (version 1.1.1) and Lumi package in R
Log2 transformation and quantile normalization
|
| |
|
| Submission date |
Nov 08, 2012 |
| Last update date |
Mar 24, 2015 |
| Contact name |
Tiziano Pramparo |
| Organization name |
Travere
|
| Department |
R&D
|
| Street address |
3611 Valley Centre Drive
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92132 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE42133 |
Disrupted functional neworks in autism underlie early brain maldevelopment and provide accurate classification |
| GSE111176 |
Transcriptional organization of autism spectrum disorder |
|
