|
| Status |
Public on Nov 09, 2012 |
| Title |
Mouse embryo, 20 hours, uninjected (for Nanog experiment), biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
Preimplantation embryos (uninjected) collected at 20 hours post-injection
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6D2F1
developmental stage: preimplantation embryo
condition: Uninjected
time (post-injection): 20 hours
|
| Treatment protocol |
Embryos were injected with either a morpholino targeting Oct4, Sall4, or Nanog or a control morpholino targeting a sequence that does not exist in the mouse genome or transcriptome.
|
| Growth protocol |
Embryos were cultured in drops of Human Tubal Fluid with 10% serum supplement (SAGE In-Vitro Fertilization Inc.) under mineral oil (Sigma) in 5% CO2 at 37oC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the PicoPure RNA Isolation Kit (Arcturus) according to manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
The RNA was reverse transcribed and amplified using the WT-Ovation One-Direct Amplification System (NuGEN). The cDNA was then fragmented and labelled using the FL-Ovation cDNA Biotin Module (NuGEN).
|
| |
|
| Hybridization protocol |
The arrays were hybridized in an Affymetrix GeneChip Hybridization Oven 640 for 40 hours.
|
| Scan protocol |
The arrays were scanned using a Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data
Mouse_20h_UI(NN)_R3
|
| Data processing |
The data were processed using dChip.
|
| |
|
| Submission date |
Nov 08, 2012 |
| Last update date |
Nov 09, 2012 |
| Contact name |
Kin Fai Au |
| E-mail(s) |
[email protected]
|
| Organization name |
Stanford
|
| Department |
Statistics
|
| Street address |
James H. Clark Center, 1st Floor East, 318 Campus Drive,
|
| City |
Stanford |
| ZIP/Postal code |
CA 94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE42135 |
Expression data from preimplantation mouse embryos |
|
