|
| Status |
Public on May 22, 2013 |
| Title |
primary abdominal NB tumor TTT009 |
| Sample type |
RNA |
| |
|
| Source name |
primary abdominal NB tumor
|
| Organism |
Mus musculus |
| Characteristics |
tissue: primary abdominal NB tumor
genotype/variation: deficient caspase-8, with metastasis to the bone marrow
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from mouse tumors using Trizol reagent (Invitrogen) with two washes with 75% ethanol. RNA quality was confirmed by analysis on the Agilient 2100 Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
100ng of total RNA were labeled with Cy3 according to Agilent's labeling protocol
|
| |
|
| Hybridization protocol |
Microarray hybridization was performed at 55°C for 20 hours in Agilent's microarray hybridization chambers, followed by wash procedures according to the manufacturer’s recommended protocols
|
| Scan protocol |
Microarray was scanned in an Agilent scanner at 5μm resolution, and the array data was extracted with Agilent feature extraction software using the miRNA_105_Jan09 protocol
|
| Description |
Biological replicate 3 of 7, deficient caspase-8, with metastasis
|
| Data processing |
Agilent Feature Extraction Software (version 10.5.1.1) was used, followed by a log transformation, percentile normalization across probes, and scaling through the samples, prior to actual analysis.
|
| |
|
| Submission date |
Nov 13, 2012 |
| Last update date |
May 22, 2013 |
| Contact name |
Yong-Dong Wang |
| E-mail(s) |
[email protected]
|
| Phone |
9015954224
|
| Organization name |
St. Jude Children's Research Hospital
|
| Street address |
262 Danny Thomas Pl, MS1135
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38134 |
| Country |
USA |
| |
|
| Platform ID |
GPL16275 |
| Series (1) |
| GSE42254 |
TH-MYCN Mice with Caspase-8 Deficiency Develop Advanced Neuroblastoma with Bone Marrow Metastasis |
|
