|
| Status |
Public on Nov 14, 2012 |
| Title |
Uninfected TG at 5 d.p.i., rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Uninfected TG mouse brain at 5 d.p.i.
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
genotype/variation: hsp70 transgenic
tissue: brain (right cerebral hemisphere)
age: 7 days
treatment: PBS control
time post-infection: 5
|
| Treatment protocol |
Mice 42 h of age received an intracranial inoculation of 4x10(4) PFU Edmonston MeV (infected) or an equivalent volume of phosphate-buffered saline (uninfected) in the left cerebral hemisphere.
|
| Growth protocol |
H-2(d) congenic C57BL/6 mice, with (TG) or without (NT) the human hsp70 transgene driven by the neuron-specific enolase promoter, were used in these studies.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total brain RNA was isolated from the right cerebral hemisphere, 5 or 10 d.p.i., using the RNeasy lipid tissue mini-kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
2ug RNA was enzymatically labeled using the BioArray High Yield RNA Transcript Labeling kit (Enzo).
|
| |
|
| Hybridization protocol |
Samples were hybridized using Herring sperm DNA, acetylated BSA (50mg/mL), BioArray Eukaryotic Hybridization Controls (Enzo Life Sciences); heat 65C for 5 min, 90C for 5 min, 45C for 5 min, centrifuge top speed for 5 min; hybridize 16 h at 45C, 60rpm; Fluidics script EukGE-WS2v4_450.
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G using GCOS (GeneChip Operating Software).
|
| Description |
MO-Tg U2
Gene expression data from PBS-injected TG mice brain at 5 d.p.i.
|
| Data processing |
Raw data were analyzed through Bioconductor package affy. The RMA method was used as the background-correction, normalization, and probe summarization method.
|
| |
|
| Submission date |
Nov 13, 2012 |
| Last update date |
Nov 14, 2012 |
| Contact name |
Lianbo Yu |
| Organization name |
The Ohio State University
|
| Department |
Center for Biostatistics
|
| Lab |
320 Lincoln Tower
|
| Street address |
1800 Cannon Drive
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE42264 |
Hsp70 and a Novel Axis of Type 1 Interferon-Dependent Antiviral Immunity in the Measles Virus-Infected Brain |
|
