|
| Status |
Public on Dec 10, 2012 |
| Title |
Healthy Living donor [H4-Glom-LD7] |
| Sample type |
RNA |
| |
|
| Source name |
Kidney Biopsy from Human Healthy Living donor
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Glomeruli from kidney biopsy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy mini kit from Qiagen, following the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix). GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
| |
|
| Hybridization protocol |
According to the Affymetrix Expression Analysis Technical Manual.
|
| Scan protocol |
GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
| Description |
Gene expression data from the glomeruli of human pre-transplant living donor control kidney
|
| Data processing |
The data were analyzed using GenePattern pipeline: RMA method and Human Entrez Gene custom CDF annotation version 10 was used for the normalization. Each of the hybridization was normalized seperately and the VALUE column contains the batch-corrected data using Combat.
|
| |
|
| Submission date |
Nov 29, 2012 |
| Last update date |
Dec 11, 2012 |
| Contact name |
Viji Nair |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Michigan
|
| Street address |
1152 West Medical Centre Dr.
|
| City |
Ann Arbor |
| ZIP/Postal code |
48331 |
| Country |
USA |
| |
|
| Platform ID |
GPL14663 |
| Series (2) |
| GSE37460 |
Cross-species transcriptional network analysis defines shared inflammatory responses in murine and human lupus nephritis [Glomeruli] |
| GSE37463 |
Cross-species transcriptional network analysis defines shared inflammatory responses in murine and human lupus nephritis |
|
