|
| Status |
Public on Oct 17, 2013 |
| Title |
Ischemic cardiomyopathy_G33 |
| Sample type |
RNA |
| |
|
| Source name |
Ischemic cardiomyopathy
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
tissue: heart
tissue: Transmural sample taken from near the apex of the left ventricle
|
| Treatment protocol |
Transmural samples were taken from near the apex of the left ventricle and stored 4C for a maximum of 6 h from the time of coronary circulation loss. Samples were stored at -80C until the RNA extraction was performed.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using a RNeasy fibrous Tissue Mini kit from Qiagen according to the manufacturer´s intructions.
|
| Label |
biotin
|
| Label protocol |
Biotinilated cRNA were prepared according to the standard Affymetrix protocol from Ambion WT Expression Sense Target Protocol kit.
|
| |
|
| Hybridization protocol |
GeneChips were hybridized using the Gene Chip Scanner 7G System platform.
|
| Scan protocol |
GeneChips were scanned using the Gene Chip Scanner 7G System platform. GeneChip Comand Console software was used for initial image processing. Affymetrix Expression ConsoleTM software provided quality control and probe set summarization to attain gene-level signal data.
|
| Description |
Gene expression data from ischemic heart tissue
G_33
|
| Data processing |
The data were analyzed with Partek Software, version 6,3 Copyright 2008, using Affymetrix default analysis settings.
Normalization and summarization of the data (background correction, normalization, log 2, and probe summarization with the RMA algorithm)
|
| |
|
| Submission date |
Dec 17, 2012 |
| Last update date |
Oct 17, 2013 |
| Contact name |
Maria Micaela Molina Navarro |
| Organization name |
Health Research Institute of the Hospital La Fe
|
| Street address |
Avenida Campanar, 21
|
| City |
Valencia |
| ZIP/Postal code |
46009 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE42955 |
Expression data from human heart |
|
