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Status
Public on Apr 20, 2006
Title
13130403
Sample type
RNA
Channel 1 Source name
190405+
Organism
Actinobacillus pleuropneumoniae Characteristics
serotype 1, strain s4074
Extracted molecule
total RNA
Label
Cy5
Channel 2 Source name
190405-
Organism
Actinobacillus pleuropneumoniae Characteristics
serotype 1, strain s4074
Extracted molecule
total RNA
Label
Cy3
Description
Competitive hybridization of cDNA generated from A. pleuropneumoniae sero 1 strain s4074 RNA extracted in iron-rich or iron-restricted conditinos
Data processing
Image processing software: SpotFinder v.3.1.1. Net median intensities were normalized using Lowess algorithm (MIDAS) and log2 ratios were generated
Submission date
Apr 14, 2006
Last update date
Apr 19, 2006
Contact name
Vincent Deslandes
E-mail(s)
[email protected] Organization name
Université de Montréal - Faculté de Médecine Vétérinaire
Department
Groupe de Recherche sur les Maladies Infectieuses du Porc
Street address
3200 rue Sicotte
City
St-Hyacinthe
State/province
Quebec
ZIP/Postal code
J2S7C6
Country
Canada
Platform ID
GPL3648 Series (1)
GSE4674 Transcript profiling in A. pleuropneumoniae under iron restriction
Data table header descriptions ID_REF IA Net Median Intensity Ch1 (raw - bckg) measured by SpotFinder, prior to normalization with Lowess
IB Net Median Intensity Ch2 (raw - bckg) measured by SpotFinder, prior to normalization with Lowess
FLAG MIDAS One Bad Channel Tolerance Policy parameter on how to deal with one-bad-channel spots: Generous option (v2.19 manual). 0 = good spots, 1 = 1 bad channel, 2 = ignored spots.
VALUE same as UNF_VALUE but with flagged values removed
UNF_VALUE After Lowess normalization, normalized log2 iron-restricted/iron-rich ratio (Ch2/Ch1)
Data table ID_REF IA IB FLAG VALUE UNF_VALUE 1
2014
1752
0
-0.16688378
-0.16688378
2
1857
1823
0
-0.0015546145
-0.0015546145
3
25647
28651
2
-0.411685
4
16674
30365
2
0.23133838
5
12505
8042
0
-1.5675321
-1.5675321
6
0
0
2
0.0
7
2201
2213
0
0.054035056
0.054035056
8
1978
1963
0
0.07045871
0.07045871
9
1733
1763
0
0.06669789
0.06669789
10
1712
0
1
0.07231418
0.07231418
11
1677
1947
0
0.23743013
0.23743013
12
0
2123
1
0.0
0.0
13
2332
2534
1
0.031816445
0.031816445
14
2903
2851
0
-0.3548523
-0.3548523
15
2548
2988
2
-0.05070697
16
2294
2834
2
0.1372748
17
2850
6619
2
0.06725217
18
3057
4998
2
-0.27297917
19
1223
2419
0
1.0441439
1.0441439
20
2472
2131
0
-0.20806798
-0.20806798
4608 168 Kbytes .Supplementary file Size Download File type/resource GSM105440.txt.gz
131.2 Kb
(ftp) (http) TXT
GSM105440_mev.txt.gz
121.0 Kb
(ftp) (http) TXT
