|
| Status |
Public on Dec 27, 2012 |
| Title |
Thymus wt 4 |
| Sample type |
RNA |
| |
|
| Source name |
Thymus
|
| Organism |
Mus musculus |
| Characteristics |
tissue: thymus
genotype: RelB+/-
age: 8 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was generated from 500 ng of total RNA using the Illumina TotalPrep cRNA Amplification Kit® (ABI)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
4991825024_G
|
| Data processing |
Array data were processed using the Illumina GenomeStudio software, transformed by variance stabilization transformation and normalized by robust spline normalization using Lumi.
|
| |
|
| Submission date |
Dec 26, 2012 |
| Last update date |
Dec 27, 2012 |
| Contact name |
Brendan John O'Sullivan |
| Organization name |
The University of Queensland Diamantina Institute Translational Research Institute
|
| Street address |
Level 6, 37 Kent Street Woolloongabba
|
| City |
Brisbane |
| State/province |
Queensland |
| ZIP/Postal code |
4102 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE43154 |
Comparison of RelB-/- and RelB+/- thymi |
|
