|
| Status |
Public on Jan 15, 2013 |
| Title |
mammary tumor after orthotopic injection of 6dt1 cells expressing negative control rep2 |
| Sample type |
RNA |
| |
|
| Source name |
mammary tumor
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB/N
tissue: mammary tumor
treatment: orthotopic injection of 6dt1 cells expressing negative control
|
| Treatment protocol |
not applicable
|
| Growth protocol |
Mammary tumors were collected 30 days after orthotopic injection of 6dt1 cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were snap frozen in liquid N2. Total RNA was isolated using RNAeasy Kit (Qiagen). RNA (100 ng) was reverse transcribed and amplified using WT Expression Kit (Ambion).
|
| Label |
Affymetrix proprietary DNA Labeling Reagent that is covalently linked to biotin.
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the GeneChip WT Terminal Labeling and Controls Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized in the GeneChip Hybridization Oven 645 while shaking at 60 rpm at 45C for 16 hrs.
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
Mammary tumor RNA collected 30 days after orthotopic injection of 6dt1 cells in female FVB/N mice.
|
| Data processing |
Data were collected using the GeneChip Command Console Software (AGCC).
MoEx-1_0-st-v1.r2.pgf
MoGene-1_0-st-v1.na32.mm9.transcript
|
| |
|
| Submission date |
Jan 14, 2013 |
| Last update date |
Jan 15, 2013 |
| Contact name |
Natalie Goldberger |
| Organization name |
NCI
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL10740 |
| Series (2) |
| GSE43485 |
Expression profiling in miR-290 6dt1 mammary tumors |
| GSE43487 |
Mammary tumors |
|
