|
| Status |
Public on Apr 24, 2006 |
| Title |
C57BL/6 heart left ventrical (LAD ligation 30 day) |
| Sample type |
RNA |
| |
|
| Source name |
30 day LAD ligated heart from C57BL/6 strain
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6
Gender: Male
Age: 2 to 4 months
Tissue: Heart, left ventrical
|
| Treatment protocol |
LAD ligation for 30 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from C57BL/6 strain mouse left ventrical with 30 day LAD ligated heart.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Apr 24, 2006 |
| Last update date |
Apr 24, 2006 |
| Contact name |
Daniel J Garry |
| Organization name |
LILLEHEI HEART INSTITUTE
|
| Department |
Cancer & Cardiovascular Research Center
|
| Street address |
2231 6th Street SE, 4-156 CCRB, 2812A
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE4710 |
Expression data from C57BL/6 and MRL/MpJ heart left ventrical with LAD ligation |
|
