|
| Status |
Public on Jan 18, 2013 |
| Title |
Trib1-/- GMP |
| Sample type |
RNA |
| |
|
| Source name |
Trib1-/- GMP
|
| Organism |
Mus musculus |
| Characteristics |
strain: mixed background C57BL6J/129SV
genotype/ variation: Trib1-/- unstimulated
cell type: granulocyte monocyte progenitor
|
| Treatment protocol |
no treatment
|
| Growth protocol |
Mouse progenitor cells were sorted. No treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared by NuGEN Ovation system according to the manufactuerer's inscrution.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Gene CHIP scanner 3000.
|
| Data processing |
The data were analyzed with RMA using R program
|
| |
|
| Submission date |
Jan 16, 2013 |
| Last update date |
Jan 23, 2013 |
| Contact name |
Takashi Satoh |
| E-mail(s) |
[email protected]
|
| Phone |
81-6-6879-8303
|
| Fax |
81-6-6879-8305
|
| Organization name |
Osaka university
|
| Department |
RIMD
|
| Lab |
Host Defense
|
| Street address |
3-1 Yamada-oka
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE43563 |
Expression data of GMP and MDP from wild-type and Trib1-/- mice |
|
