|
| Status |
Public on Jan 18, 2013 |
| Title |
HCT116 3 days hypoxia exp B |
| Sample type |
RNA |
| |
|
| Source name |
HCT116
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: colon cancer
cell line: HCT116 colon cancer cells
treatment protocol: hypoxia
|
| Treatment protocol |
Serum starved HCT116 were placed under normoxia or hypoxia for 72 hours.
|
| Growth protocol |
Subconfluent HCT116 were washed with PBS once and media without serum was added for 24 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen extraction kits.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
HCT116 3 days hypoxia exp B.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jan 18, 2013 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Nicole Fer |
| E-mail(s) |
[email protected]
|
| Organization name |
Frederick National Laboratory for Cancer Research
|
| Street address |
PO Box B
|
| City |
Frederick |
| State/province |
MD |
| ZIP/Postal code |
21702 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE43608 |
Gene expression profile of HCT116 exposed to chronic hypoxia |
|
