|
| Status |
Public on Feb 01, 2013 |
| Title |
pupal wing ~80h APF, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila melanogaster pupal wings, ~80h APF
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: Oregon R
tissue: pupal wings
|
| Treatment protocol |
Pupae were manually extracted from the pupal case, the pupal membrane surrounding the wings was removed with tweezer and the wings were dissected and directly collected and collected in a small drop of water in a tube on dry ice.
|
| Growth protocol |
Flies were grown at 25C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Wings were mechanically discrupted in Trizol using aTissueTearor (to crack the cuticle surrounding the cells) and then processed for RNA extraction according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
|
| |
|
| Hybridization protocol |
biotin-labeled cRNA was hybridized overnight to D. melanogaster GeneChip in the AFX HybOven480 and post processed on the automated Fluidics450 Station
|
| Scan protocol |
GeneChips were scanned on the GC 3000 G7 Scanner
|
| Description |
Gene expression data from pupal wings ~80h APF
|
| Data processing |
The data was analyzed using BioConductor (MAS 5.0 evaluations and RMA calculations)
|
| |
|
| Submission date |
Jan 23, 2013 |
| Last update date |
Feb 02, 2013 |
| Contact name |
Benjamin Prudhomme |
| E-mail(s) |
[email protected]
|
| Organization name |
IBDM-CNRS
|
| Street address |
Parc scientifique de Luminy case 907
|
| City |
Marseille |
| ZIP/Postal code |
13288 |
| Country |
France |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE43673 |
Expression data from D. melanogaster pupal wings |
|
