|
| Status |
Public on Feb 06, 2014 |
| Title |
MB231 PIAS1 shRNA-SCM |
| Sample type |
RNA |
| |
|
| Source name |
MDA-MB231 PIAS1 shRNA cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB231
treatment: PIAS1 shRNA
media: SCM
|
| Growth protocol |
MDA-MB231 Control shRNA and PIAS1 shRNA2 cells were cultured in DMEM plus 10% FBS (DMEM) or SCM for 30 h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from MDA-MB231 PIAS1 shRNA cells grown in SCM for 30 h.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Feb 01, 2013 |
| Last update date |
Feb 06, 2014 |
| Contact name |
BIN LIU |
| E-mail(s) |
[email protected]
|
| Organization name |
UCLA
|
| Street address |
10833 Le Conte Avenue
|
| City |
Los Angeles |
| ZIP/Postal code |
90024 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE44024 |
Effect of PIAS1 on gene expression |
|
