|
| Status |
Public on Apr 26, 2013 |
| Title |
Col 3 Row 2-Array3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
liquid culture
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype: Adaptive Mutant PG-132
|
| Growth protocol |
Evolutionary engineering of Saccharomyces cerevisiae for enhanced tolerance to hydrolysates of lignocellulosic biomass. The cells were grown in YNB minimal media (No glucose), and in 1g/mL Hydrolysates biomass
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using ZR Fungal/Bacterial RNA MiniPrep™ (Zymo) kit
|
| Label |
cy5
|
| Label protocol |
5 µg of cDNA was labeled using Cy3- and Cy5- mono-Reactive Dye Pack (GE Healthcare)
|
| |
|
| Channel 2 |
| Source name |
liquid culture
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype: reference GFP
|
| Growth protocol |
Evolutionary engineering of Saccharomyces cerevisiae for enhanced tolerance to hydrolysates of lignocellulosic biomass. The cells were grown in YNB minimal media (No glucose), and in 1g/mL Hydrolysates biomass
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using ZR Fungal/Bacterial RNA MiniPrep™ (Zymo) kit
|
| Label |
cy3
|
| Label protocol |
5 µg of cDNA was labeled using Cy3- and Cy5- mono-Reactive Dye Pack (GE Healthcare)
|
| |
|
| |
| Hybridization protocol |
Samples were for hybridization by adding lyophilized 10X Blocking Agent (supplied with Agilent Oligo aCGH Hybridization Kit), following manufacture's protocol. The samples were hybridazed in a Agilent's Saccharomyces cerevisiae Gene Expression Microarray, 8x15K G4813A
|
| Scan protocol |
The arrays were scanned using the GenePix 4100A Microarray Scanner
|
| Description |
Sample 22
Microarray3.gpr
|
| Data processing |
The arrays were scanned using the GenePix 4100A Microarray Scanner and GenePix Pro 6.0 software (Molecular Devices) for image analysis.The Microarray Data Analysis System (MIDAS) software was used to normalize the data using LOWESS based normalization algorithm.
|
| |
|
| Submission date |
Feb 05, 2013 |
| Last update date |
Apr 26, 2013 |
| Contact name |
Maria Priscila Almario |
| E-mail(s) |
[email protected]
|
| Organization name |
Texas A&M University
|
| Department |
Chemical Engineering
|
| Lab |
Katy Kao Lab
|
| Street address |
3122 TAMU
|
| City |
College Station |
| State/province |
Texas |
| ZIP/Postal code |
77843-3122 |
| Country |
USA |
| |
|
| Platform ID |
GPL9825 |
| Series (1) |
| GSE44085 |
Evolutionary engineering of Saccharomyces cerevisiae for enhanced tolerance to hydrolysates of lignocellulosic biomass Samples vs Reference |
|
