|
| Status |
Public on Jul 08, 2013 |
| Title |
White Cold rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse white adipose tissue
|
| Organism |
Mus musculus |
| Characteristics |
treatment: 22 C, 48 hours + 8 C, 24 hours
tissue: White Adipose
strain: C57BL/6
Sex: Female
|
| Treatment protocol |
Warm group treated at 22 C for 72 hours. Cold group treated at 22 C for 48 hours then 8 C for 24 hours.
|
| Growth protocol |
Mice were housed individually in cages measuring 48x14x13 cm, 16 hour light 8 hour dark cycle, access to to beeding material, standard mouse chow diet.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Tri Reagent (Sigma)
|
| Label |
Biotin
|
| Label protocol |
RNA samples were labeled and amplified using the Illumina RNA amplification kit as per mnufacturer's instructions
|
| |
|
| Hybridization protocol |
Standard Illumina hybridisation protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
15
|
| Data processing |
All data was processed using Genespring GX ver 10 (Agilent Technologies)
|
| |
|
| Submission date |
Feb 07, 2013 |
| Last update date |
Jul 08, 2013 |
| Contact name |
Neil Graham |
| E-mail(s) |
[email protected]
|
| Phone |
01159516373
|
| Organization name |
University of Nottingham
|
| Department |
Plant and Crop Sciences
|
| Lab |
Broadley
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| State/province |
Leicestershire |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10242 |
| Series (1) |
| GSE44138 |
Cold-induced changes in gene expression in brown adipose tissue, white adipose tissue and liver |
|
