|
| Status |
Public on May 03, 2013 |
| Title |
Id2-GFPhigh Repl2 |
| Sample type |
RNA |
| |
|
| Source name |
Purified Id2-GFPhigh DbNP366-specific CD8+ T cells
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: Id2gfp/gfp
tissue: Spleen
cell type: Db-NP366 specific CD8+ T cells
levels of gfp: high
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using a Qiagen RNeasy Mini Kit followed by DNase treatment . Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 5
|
| Data processing |
The data was background corrected, quantile normalised and log2 transformed (with shift = 16) using neqc function from limma package (v.3.14.4) in R /Bioconductor (v.2.15.2).
|
| |
|
| Submission date |
Feb 07, 2013 |
| Last update date |
May 03, 2013 |
| Contact name |
Moshe Olshansky |
| E-mail(s) |
[email protected]
|
| Organization name |
Walter & Eliza Hall Institute of Medical Research
|
| Department |
Bioinformatics
|
| Lab |
Speed
|
| Street address |
1G Royal Pde
|
| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE44140 |
Whole genome mRNA analysis of virus-specific CD8+ T cells expressing different levels of Id2-GFP following influenza virus infection |
|
