|
| Status |
Public on Apr 22, 2013 |
| Title |
Kc cells using RNAi against H1_a |
| Sample type |
RNA |
| |
|
| Source name |
Kc Cells
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell type: Kc Cells
RNAi against: linker histone H1
|
| Treatment protocol |
Kc cells were treated with 20mg of dsRNA targeted against GFP or H1 for 1 hour without serum on day 0, 2, and 4. Cells were washed and fresh Scheidner's media was added. RNA was subsequently isolated on day 6. Transgenic flies used for isolation of salivary glands were described in X Lu et al. Genes Dev 2009.
|
| Growth protocol |
Flies were crossed at 18o and knockdown was achieved by incubating at 29o from the embryo stage. Kc cells were grown at 25o in Scheidner's media containing 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with Trizol reagent according to manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Labeling was performed as suggested by Affymetrix by the AECOM microarray facility.
|
| |
|
| Hybridization protocol |
Standard Affymetrix guidelines.
|
| Scan protocol |
Samples were scanned by the AECOM microarray facility as suggested by Affymetrix.
|
| Description |
KcH1a
Cells were treated with RNAi targeting H1 on day 0, 2, and 4. RNA was collected on day 6.
|
| Data processing |
The raw data (CEL files) from each microarray was normalized by RMA methods in GeneSpring GX software. The transformed signal intensities were averaged for biological replicates, and the mean value was used to compute the fold expression change.
|
| |
|
| Submission date |
Feb 07, 2013 |
| Last update date |
Apr 22, 2013 |
| Contact name |
Sandeep Nagaraj Wontakal |
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Cell Biology
|
| Lab |
Dr. Art Skoultchi
|
| Street address |
1300 Morris Park Ave Chanin 402
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE44148 |
Analysis of Drosophila salivary glands and Kc cells with depleted levels of linker histone H1 |
| GSE44398 |
Analysis of Drosophila salivary glands and Kc cells with depleted levels of linker histone H1 [Affymetrix Expression] |
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