|
| Status |
Public on May 12, 2006 |
| Title |
Substantia Nigra Control Sample4 RMA, MAS 5, dChip |
| Sample type |
RNA |
| |
|
| Source name |
Pooled Substantia Nigra C57BL/6 Saline injection
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6
Gender: Male
Age: 10 Weeks
TissueL Substantia Nigra(SN) dissected bilaterally from six like-treated mice.
|
| Treatment protocol |
Mice received 4 injections of 0.9% sterile saline, of equal volume to treatment group. The first and second doses were separated by 16 hr. Mice were killed 24hr or 7d after injection. The SN was dissected bilaterally from six like-treated mice, pooled and RNA was prepared from Trizol homogenates per the manufacturer's protocol. (Invitrogen)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Biotinylated UTP & CTP
|
| Label protocol |
Enzo IVT protocol
|
| |
|
| Hybridization protocol |
Affymetrix Standard
|
| Scan protocol |
Affymetrix Standare using U74A mask file
|
| Description |
MAS 5.0, scaled to 500; Affymetrix data Mining Tool 3.0; dChip was used to determine differentially expressed genes (MBEI); Statistical Analysis of Microarrays(SAM), as described in Miller et al. 2004.
|
| Data processing |
Raw intensity data acquired from Affymetrix platform were RMA Normalized.
|
| |
|
| Submission date |
May 05, 2006 |
| Last update date |
Sep 12, 2006 |
| Contact name |
Howard J Federoff |
| Phone |
(585) 273-2190
|
| URL |
http://www.urmc.rochester.edu/gebs/faculty/Howard_Federoff.htm
|
| Organization name |
University of Rochester
|
| Department |
Center for Aging and Developmental Biology
|
| Lab |
Federoff
|
| Street address |
601 Elmwood Ave Box 645
|
| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
| |
|
| Platform ID |
GPL32 |
| Series (1) |
| GSE4788 |
Dysregulation of Gene Expression in the 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Lesioined Mouse Substantia Nigra |
|
