|
| Status |
Public on Sep 06, 2013 |
| Title |
nub1, gut, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
nub1, gut
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: gut
strain: nub1 b1 pr1
genotype/variation: nub1 mutant
age: 7-12 days old female flies
|
| Treatment protocol |
No treatment was used prior to RNA extraction.
|
| Growth protocol |
Prior to analysis flies were maintained in mixed female/male populations on standard cornmeal agar medium in vials and kept in humid culture rooms at 25°C with a 12 h light/12 h dark cycle. 7-12 days old female flies were used. Pools of 20 flies were used per sample replicate.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted as described {Davis, 2011}, from dissected guts and from the rest of the fly minus gut and head (carcass) of 7-12 days old female flies. Tissues from three independent pools of 20 flies were used and analyzed as biological replicates.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
As recomended from the manufacturer (Affymetrix)
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from wild type and nub1 gut or carcass, respectively.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method in Expression Console
|
| |
|
| Submission date |
Feb 11, 2013 |
| Last update date |
Sep 07, 2013 |
| Contact name |
Jessica Margareta Lindvall |
| E-mail(s) |
[email protected]
|
| Organization name |
SciLifeLab. Stockholm University
|
| Department |
NBIS
|
| Street address |
Tomtebodavägen 23A
|
| City |
Solna |
| ZIP/Postal code |
11111 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE44234 |
Pdm1/nub repression of innate immunity |
|
