|
| Status |
Public on Feb 17, 2013 |
| Title |
Inf14tot |
| Sample type |
RNA |
| |
|
| Source name |
Huh7 cells infected with HCV JFJ1 - replicate 4
|
| Organism |
Homo sapiens |
| Characteristics |
infection: Infected with HCV JFJ1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were then extracted with Trizol, and precipitated with isopropanol (Sigma). The quality of RNAs was checked on an Agilent 2100 Bioanalyser.
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeling was performed with the Agilent kit, from 100 ng of total RNA.
|
| |
|
| Hybridization protocol |
Each Cy3-labeled RNA was hybridized on an Agilent 8x15k human miRNA microarray slide.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner.
|
| Description |
Total RNA from Huh7 cells infected with HCV JFJ1 - replicate 4
|
| Data processing |
The rawdata have been normalized with the quantile method (R software).
|
| |
|
| Submission date |
Feb 17, 2013 |
| Last update date |
Feb 17, 2013 |
| Contact name |
Cyrille Féray |
| Organization name |
Faculté de médecine de Nantes
|
| Lab |
EA4271
|
| Street address |
1 rue Gaston Veil
|
| City |
Nantes |
| ZIP/Postal code |
44000 |
| Country |
France |
| |
|
| Platform ID |
GPL13264 |
| Series (2) |
| GSE44369 |
MiRNA analysis during hepatitis C virus infection |
| GSE44370 |
mRNA and miRNA analysis during hepatitis C virus infection |
|
