gene reported: wild-type strain: CD-1 Sex: Male developmental stage: gd 12 theiler stage: 20 somite count: NA developmental landmark: NA
Treatment protocol
pool size: 5 Pooled sample: Yes Dissection Method: Whole organ excision Experimental Design: CD-1 mice are time-mated within a 2 hour window. Pregnant CD-1 mice are euthanized by standard carbon dioxide asphyxiation. All fetuses are killed by decapitation with a scalpel. Fetal gonads are removed from fetuses at gestation days 11, 12, 14, 16, 18, and postnatal day 2, snap frozen in liquid nitrogen and stored at -80ºC.
Extracted molecule
total RNA
Extraction protocol
Gaido protocol: 'RNA isolation from whole testes'
Label
Biotin
Label protocol
-
Hybridization protocol
Affymetrix standard protocol (701021 Rev 4) Amount labeled target hybridization to array: 10 ug
