|
| Status |
Public on Apr 02, 2013 |
| Title |
SKNAS_Sphere_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Neuroblastoma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell origin: Neuroblastoma
culture condition: Sphere
pretreatment: None
|
| Treatment protocol |
SKNAS iCSC was first treated with 5AdC (2.5 uM) for five days as monolayer culture and then transferred to the serum free medium to form spheroid culture.
|
| Growth protocol |
SKNAS Monolayer was grown as monolayer culture. SKNAS Sphere was grown as spheroid culture in a serum free medium. SKNAS iCSC was grown as spheroid culture in a serum free medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen RNeasy kit.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated amplified RNA (aRNA) was prepared from 200 ng total RNA using the standard protocol of the Ambion MessageAmp Premier Kit.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133_P_2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, 7G.
|
| Data processing |
Probe-level data in CEL files was normalized using GCRMA method implemented in Partek Genomics Suite to get gene-level expression measurements.
|
| |
|
| Submission date |
Feb 21, 2013 |
| Last update date |
Apr 02, 2013 |
| Contact name |
Eric Rappaport |
| E-mail(s) |
[email protected]
|
| Organization name |
Children's Hospital of Philadelphia
|
| Street address |
3615 Civic Center Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE44537 |
Transient treatment with epigenetic modifiers yields stable neuroblastoma stem cells resembling aggressive large-cell neuroblastomas |
|
