|
| Status |
Public on May 01, 2013 |
| Title |
HG0242_F_liver |
| Sample type |
RNA |
| |
|
| Source name |
WSB(dam) x PWK(sire) F1,liver
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
age: 23 days old
|
| Treatment protocol |
All animals were bred at UNC from mice that were less than six generations removed from founders acquired from the Jackson Laboratory (Bar Harbor, ME).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from ~25 mg of tissue powder using automated instrumentation (Maxwell 16 Tissue LEV Total RNA Purification Kit, Promega, Madison, WI).
|
| Label |
biotin
|
| Label protocol |
WT Terminal Labeling and Controls Kit (part #901525)
|
| |
|
| Hybridization protocol |
Total RNA were hybridized to Affymetrix Mouse Gene 1.1 ST 96-Array Plate arrays using a GeneTitan instrument from Affymetrix according to the manufacturer’s protocols
|
| Scan protocol |
Affymetrix GeneTitan Instrument
|
| Description |
gene expression data
|
| Data processing |
Robust multiarray average method (RMA) implemented in the Affymetrix gene expression console with default settings (median polish and sketch-quantile normalization) to estimate normalized expression levels of transcripts
|
| |
|
| Submission date |
Feb 21, 2013 |
| Last update date |
May 01, 2013 |
| Contact name |
Yunjung Kim |
| E-mail(s) |
[email protected]
|
| Organization name |
UNC at Chapel Hill
|
| Street address |
120 Mason farm road
|
| City |
chapel hill |
| State/province |
North Carolina |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE44555 |
Multiple tissue expression data from inbreds and F1 of CAST, PWK, and WSB |
|
