|
| Status |
Public on May 30, 2013 |
| Title |
ST045849 24 hours_2 |
| Sample type |
RNA |
| |
|
| Source name |
ST045849 24 hours_2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LNCaP
|
| Treatment protocol |
Cells were plated directly on siRNA or drug / vehicle
|
| Growth protocol |
LNCaP cells were maintained in RPMI media supplemented with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was collected by illustraMiniSpin (GE Healthcare) according to manufacturer’s instructions. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Labelling was performed with Illumina® TotalPrep™-96 RNA Amplification Kit according to manufacturer's instructions
|
| |
|
| Hybridization protocol |
Hybridisation of cRNA onto the Illumina HumanHT-12 v.4 Expression BeadChips (Illumina Inc, California, USA) was carried out as per manufacturer's instructions
|
| Scan protocol |
After scanning, the iScan software extracts signal intensities and saves all files for each BeadChip. Raw data is imported to Illumina’s GenomeStudio software V2011.1, Gene Expression module v. 1.9.0. Here the quality of each array and scan is investigated, and text files with both non-normalized and quantile normalized data exported.
|
| Description |
H14
|
| Data processing |
Data extraction, quantile normalization and initial quality control of the bead summary raw data were performed using GenomeStudio v2011.1 from Illumina and the Gene Expression module v1.9.0. The data were annotated using the HumanHT-12_V4_0_R2_15002873_B.bgx annotation file from Illumina.
|
| |
|
| Submission date |
Feb 25, 2013 |
| Last update date |
May 30, 2013 |
| Contact name |
Ian Mills |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Oslo
|
| Department |
Centre for Molecular Medicine Norway
|
| Lab |
Prostate Cancer Research Group
|
| Street address |
Gaustadalleen 21
|
| City |
Oslo |
| ZIP/Postal code |
0349 |
| Country |
Norway |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE44624 |
Glycosylation regulates the stability of c-MYC |
|
