|
| Status |
Public on Mar 21, 2014 |
| Title |
Selected mouse Ba/F3 cells transfected with pMSCV vector expressing the FIP1L1-PDGFRA oncogene |
| Sample type |
RNA |
| |
|
| Source name |
Ba/F3 cells
|
| Organism |
Mus musculus |
| Characteristics |
treatment: pMSCV-FIP1L1-PDGFRA vector transfection + selection
cell type: Mouse Pro B cells
cell line: Ba/F3
|
| Treatment protocol |
Oncogene-transformed cells were selected for growth in the absence of IL3. Before RNA extraction, cells were left untreated or incubated for 4 hours with 100 nM Glivec. Samples were washed once with PBS before RNA extraction. In control condition, cells were cultured in the presence of IL3.
|
| Growth protocol |
Ba/F3 cells were grown in DMEM medium supplied with 10% FBS, penicillin and streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total mRNA was extracted using Rneasy mini kit (Qiagen) according to manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 5 µg total mRNA.
|
| |
|
| Hybridization protocol |
Affymetrix standard
|
| Scan protocol |
Affymetrix standard
|
| Description |
Gene expression profile from selected Ba/F3 cells expressing FIP1L1-PDGFRa oncogene
|
| Data processing |
Affymetrix GCOS suite version 1.4.0 was used. The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Mar 03, 2013 |
| Last update date |
Mar 22, 2014 |
| Contact name |
Jean-Baptiste Demoulin |
| Organization name |
Université catholique de Louvain
|
| Department |
de Duve Institute
|
| Lab |
UCL - MEXP
|
| Street address |
av Hippocrate 75, B1.74.05
|
| City |
Brussels |
| ZIP/Postal code |
B-1200 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE44810 |
Ba/F3 cells expressing ETV6-PDGFRb and FIP1L1-PDGFRa treated or not with Glivec |
|
