|
| Status |
Public on Jun 02, 2006 |
| Title |
Dex-treated sample at 24hr, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
MCF10A-Myc cells treated with dexamethasone for 24hr
|
| Organism |
Homo sapiens |
| Characteristics |
Genotype: unknown
Age: 35
|
| Treatment protocol |
MCF10A-Myc cells were subjected to 72 hrs of growth factor withdrawal followed by treatment with dexamethasone for 24 hr.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from each sample was extracted using Qiagen’s RNeasy Kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on Affymetrix human genome HG-U133A genechip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000 7G..
|
| Description |
Gene expression data from MCF10A-Myc cells treated with Dex for 24 hr.
|
| Data processing |
The data were analyzed and normalized Robust Multi-array Average (RMA) algorithm.
|
| |
|
| Submission date |
May 17, 2006 |
| Last update date |
Apr 24, 2013 |
| Contact name |
Suzanne Daniela Conzen |
| E-mail(s) |
[email protected]
|
| Phone |
(773)834-2604
|
| Organization name |
The University of Chicago
|
| Department |
Medicine
|
| Lab |
Conzen Lab
|
| Street address |
5841 S. Maryland Ave, SBRI J301, MC 2115
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE4917 |
Time course microarray data following GR activation in MCF10A-Myc breast cells |
|
