|
| Status |
Public on Aug 19, 2006 |
| Title |
E14 molar DMSO 1 |
| Sample type |
RNA |
| |
|
| Source name |
E14 molar teeth, DMSO control
|
| Organism |
Mus musculus |
| Characteristics |
Strain: NMRI mouse
Age: 14 day embryo
Tissue: molar tooth germ
|
| Treatment protocol |
Tissue culture for 24 hours,
with DMSO added to the medium at 0,65%
|
| Growth protocol |
The basal culture medium was Dulbecco’s modified Eagle’s medium (DMEM) containing Glutamax-1 (Gibco BRL, Paisley, Scotland) supplemented with 10% fetal calf serum (FCS; Gibco BRL).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy Mini Kit (Qiagen). Extraction was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 11 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 16 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Murine Genome U74 Av2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400
|
| Scan protocol |
GeneChips were scanned using the
Genechip System confocal scanner (Aligent)
|
| Description |
Gene expression data from pooled samples of E14 mouse embryo tooth germs
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
May 18, 2006 |
| Last update date |
May 22, 2006 |
| Contact name |
Carin Sahlberg |
| Organization name |
University of Helsinki
|
| Department |
Institute of Dentistry
|
| Street address |
PL 63, Haartmaninkatu 8
|
| City |
Helsinki |
| ZIP/Postal code |
FIN 00014 University of Helsinki |
| Country |
Finland |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE4873 |
E14 mouse molar tooth germs cultured with/without TCDD |
|
