|
| Status |
Public on Dec 31, 2006 |
| Title |
Hsrc9 |
| Sample type |
RNA |
| |
|
| Source name |
HepG2-∆Src1
|
| Organism |
Homo sapiens |
| Characteristics |
HepG2-∆Src1 cells were generated by lentiviral infection of the HepG2 human hepatocellular carcinoma cell line with a vector coding for a small-hairpin RNA that knocks down expression of the coactivator SRC-1/NcoA-1 (nuclear receptor coactivator 1). Cells were treated with interleukin-6 for 1 h.
|
| Treatment protocol |
cells treated with 10 ng/ml interleukin-6 for 1 hour
|
| Growth protocol |
HepG2 cells were cultured in a 2:1 (v/v) mixture of Dulbecco's minimal essential medium (DMEM) and Ham's F12 medium with 10% (v/v) fetal calf serum and 1% (w/v) antibiotics (penicillin and streptomycin), at 37 degrees C and 5 % CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using the Qiagen RNeasy Mini Kit according to the manufacturer’s instructions. RNA quality was examined by the RNA 6000 LabChip Kit on the Agilent 2100 bioanalyzer.
|
| Label |
Phycoerythrin
|
| |
|
| Description |
This sample is part of a experiment series addressing the role of coactivator SRC-1/NcoA-1 for the induction of interleukin-6 (IL-6) target genes in HepG2 cells. Either control HepG2 cells or HepG2 cells manipulated to stably express an shRNA that knocks down SRC-1 expression were treated with IL-6 for 1 or 4 h or left untreated.
|
| Data processing |
Affymetrix MAS5.0
|
| |
|
| Submission date |
May 22, 2006 |
| Last update date |
May 22, 2006 |
| Contact name |
Friedemann Horn |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Leipzig
|
| Department |
Institute of Clinical Immunology and Transfusion Medicine
|
| Lab |
Molecular Immunology
|
| Street address |
Johannisalle 30
|
| City |
Leipzig |
| ZIP/Postal code |
04103 |
| Country |
Germany |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE4885 |
Role of coactivator SRC-1/NcoA-1 for IL-6 target gene induction in HepG2 cells |
|
